The goal of this work is to create antimicrobial rubber for industrial rubber products that are safe. Acrylonitrile butadiene rubber (NBR) was investigated for this reason. In a rubber mixer, rubber, ingredients, and the antimicrobial agent jojoba seed meal powder (JSMP) were mixed. The curing time, rheological characteristics, mechanical properties, dielectric properties, and antimicrobial potency of compounded rubber were examined. Scanning electron micrographs (SEM) revealed that the components were well-dispersed throughout the examined vulcanizates. According to rheology studies, there was no discernible change in the flow behaviours of the vulcanizates. It is important to keep in mind that, depending on their nature and concentration, jojoba seed meal powder (JSMP) can improve the desired physical characteristics of rubber products, including their chemical and mechanical characteristics (elongation at break, tensile strength, hardness, and equilibrium swelling). The dielectric results revealed that the presence of JSMPP caused an increase in the permittivity, dielectric loss, and conductivity of the resulting composites. It can be concluded that the presence of JSP is influential for good electrical performance for NBR/JSP compounds. Additionally, the test fungus and bacteria strains demonstrated good antimicrobial efficacy of the developed rubber vulcanizates.