This manuscript outlines the synthesis and spectral characterization of innovative Pd(II) coordination ternary metal complexes. The complexes are synthesized using aliphatic amines as the primary ligand L1 and Cephradine as the secondary ligand L2. The identification of these newly formed ternary metal complexes involves a comprehensive range of spectrophotometric and physico-chemical analyses, encompassing elemental analysis, Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1HNMR) spectroscopy, determination of melting point, conductivity measurements, mass spectrometry, and thermal analysis (TG and DTG). Furthermore, the antimicrobial activity of these complexes is evaluated against various microorganisms, including Gram-positive bacteria such as Staphylococcus aureus and Streptococcus mutans, Gram-negative bacteria such as Escherichia coli, Klebsiella pneumonia, and Pseudomonas aeruginosa, as well as fungi including Candida albicans, Aspergillus Nigar, and Aspergillus Ochraceous. To assess their efficacy, the complexes are compared to established standards in the field, namely Ampicillin and Gentamicin as antibacterial agents, and Nystatin as an antifungal agent.

Moreover, the manuscript explores the anticancer properties of the ligands and their corresponding metal complexes using viability assays against human cancer cells (MCF-7 cells).