A novel biodegradable and biocompatible conductive nanocomposite hydrogels comprising of polyaniline, polyvinyl alcohol, and copper nanoparticles (PANI/PVA/Cu) bionanocomposites were synthesized using aniline hydrochloride instead of aniline and hydrochloric acid to reduce the use of hazardous chemicals. Addition of insulating polymeric components and inorganic particles improves the physical properties of conducting polymers. A green method was used to create copper nanoparticles, the process is based on the chemical reduction of copper salt in aqueous solution utilizing ascorbic acid as a reducing and capping agent. Fourier transforms infrared spectroscopy and UV-Visible analyses were used to confirm the structural confirmation of the prepared nanocomposite hydrogels. The morphology of the prepared hydrogels was investigated by scanning electron microscopy (SEM). The X-ray diffraction (XRD) and transmission electron microscope (TEM) were used to study the morphology and particle size of nanoparticles; the obtained results demonstrate that the average particle size of nanoparticles is about 43.2 nm. LCR meter was used to measure the electrical conductivity of the samples. Additionally, the prepared hydrogels of different compositions were also assessed for their water-uptake capacity in distilled water.