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321180

Method validation for determination of eight elements in ammi-visnaga plant using graphite-atomic absorption spectrometer in Egypt

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Analytical chemistry

Abstract

In this study, we developed an analytical method for quantifying eight elements in ammi visnaga plant. The method was to quantify heavy metals including aluminum (Al), iron (Fe), chromium (Cr), selenium (Se), zinc (Zn), and potassium (K) and trace elements including copper (Cu) and manganese (Mn) using graphite atomic absorption spectroscopy technique (GFAAS). Validation of each method was performed according to ICH Q2 parameters. The methods showed linearity with R2 of the regression equation > 0.999. Limit of detection (LOD) was calculated to be 4.7, 2.7, 0.11, 0.13, 0.022, 0.063, 0.38, and 0.03 ppb for Al, Cr, Cu, Fe, Mn, Se, K, and Zn respectively. While limit of quantification (LOQ) was calculated to be 14, 8.1, 0.34, 0.40, 0.067, 0.19, 1.2, and 0.08 ppb for Al, Cr, Cu, Fe, Mn, Se, K, and Zn respectively. The methods are found to be accurate and precise by calculating the accuracy, repeatability, and intermediate precision measurements with RSD < 2. The methods were found robust by applying small changes in digestion time and different graphite furnaces. In this work, we applied the developed methods to measure the concentration of eight elements in ammi-visnaga plant which is sold in the Egyptian market. 119, 1.79, 0.55, 0.68, 1.81, 0.97, 19.8, and 0.68 ppb were the found concentrations of Al, Cr, Cu, Fe, Mn, Se, K, and Zn in the Egyptian ammi visnaga.

DOI

10.21608/ejchem.2023.228230.8402

Keywords

Ammi-visnaga, Heavy metals, trace elements, method validation, GFAAS

Authors

First Name

Mohamed A.

Last Name

Amin

MiddleName

-

Affiliation

Quality Control Manager, Pt CELL for Pharmaceutical Industries Co. 10th of Ramadan city 44629, Egypt

Email

alichem2005@yahoo.com

City

Belbies

Orcid

-

First Name

Emad M.

Last Name

Abd Halim

MiddleName

-

Affiliation

Department of Chemistry, Zagazig University, Zagazig 44519, Egypt.

Email

emadabdelhalim1979@yahoo.com

City

-

Orcid

-

First Name

Mohamed A.

Last Name

Ali

MiddleName

-

Affiliation

Department of Chemistry, School of Biotechnology, Badr University in Cairo (BUC), Badr City, Cairo, 11829, Egypt

Email

mohamed.ahmed_ali@buc.edu.eg

City

-

Orcid

0000-0002-7390-8592

First Name

Nahla A.

Last Name

Abdelshafi

MiddleName

-

Affiliation

Department of Pharmaceutical Analytical Chemistry, School of Pharmacy, Badr University in Cairo, Badr City, Cairo 11829, Egypt

Email

nahla.abdelshafy@buc.edu.eg

City

-

Orcid

0000-0003-4757-4274

Volume

67

Article Issue

4

Related Issue

45406

Issue Date

2024-04-01

Receive Date

2023-08-09

Publish Date

2024-04-01

Page Start

373

Page End

379

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_321180.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=321180

Order

321,180

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Method validation for determination of eight elements in ammi-visnaga plant using graphite-atomic absorption spectrometer in Egypt

Details

Type

Article

Created At

30 Dec 2024