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330214

Biochemical Identification of Some True Halophytes Using DNA Barcoding in the Egyptian Red Sea Coast

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Biochemistry

Abstract

Halophytes are a group of plants that thrive in high salinity. These plants are overused and endangered due to a combination of environmental factors and human behaviors. One of the ways to preserve the biological diversity of local plants is through their clear documentation to preserve the intellectual property rights of countries. Biochemical characterization using DNA barcoding is a relatively new species identification and authentication method using a short section of DNA from a specific gene or genes. In this study, ten halophytic plant species were collected from their different growth habitats along the Red Sea coast of Egypt. Then two standard chloroplast DNA loci (matK & rbcL) were used as DNA barcoding markers to document the collected species. They were successfully amplified and sequenced; the resulting barcode sequences have been deposited to the GenBank database. The sequence similarity (BLAST analysis) and the phylogenetic-based methods showed that the rbcL barcode has a high universality among the different tested taxa, whereas matK offers slightly low universality. In this context, we found that using the previously proposed barcode of rbcL alone may be difficult to differentiate between the closely related species (Tetraena alba and Tetraena coccinea). Two partial Coding Sequences (CDs) of the matK gene from Atriplex farinosa and Tetraena alba were deposited for the first time into the GenBank database (accession numbers OM164029 and OM16403, respectively). This finding is noteworthy as it represents a previously unreported addition to the available genetic information for these two plant species.

DOI

10.21608/ejchem.2023.248183.8868

Keywords

DNA barcoding, biodiversity, halophytes, Red Sea, matK, rbcL

Authors

First Name

Mohammad

Last Name

Al-Tahlawy

MiddleName

A.

Affiliation

Plant Biotechnology Department, Biotechnology Research Institute, National Research Centre (NRC), Giza, Egypt.

Email

mohammad_altahlawy@yahoo.com

City

-

Orcid

-

First Name

Mai

Last Name

Allam

MiddleName

A.

Affiliation

Plant Biotechnology Department, Biotechnology Research Institute, National Research Centre (NRC), Giza , Egypt

Email

allam99nrc@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Matter

MiddleName

A.

Affiliation

Plant Biotechnology Department, Biotechnology Research Institute, National Research Centre (NRC), Dokki, Giza 12622, Egypt.

Email

nhmatter@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Atia

MiddleName

A. M.

Affiliation

Molecular Genetics and Genome Mapping Laboratory, Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza, Egypt.

Email

matia_ageri@yahoo.com

City

-

Orcid

-

First Name

Ezeldain

Last Name

Shafee

MiddleName

-

Affiliation

Department of Chemistry, Faculty of Science, Cairo University, Giza 12613, Egypt.

Email

ezeldain@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Rady

MiddleName

R.

Affiliation

Plant Biotechnology Department, Biotechnology Research Institute, National Research Centre (NRC), Dokki, Giza 12622, Egypt.

Email

drrady1@yahoo.com

City

-

Orcid

-

First Name

Mahmoud

Last Name

Sakr

MiddleName

M.

Affiliation

President of the Egyptian Academy of scientific research and technology (ASRT) National research centre

Email

msakr@asrt.sci.eg

City

Cairo

Orcid

-

First Name

Demiana

Last Name

Hanna

MiddleName

H.

Affiliation

Department of Chemistry, Faculty of Science, Cairo University, Giza 12613, Egypt.

Email

dhelmy@sci.cu.edu.eg

City

Cairo

Orcid

https://orcid.org/00

Volume

67

Article Issue

4

Related Issue

45406

Issue Date

2024-04-01

Receive Date

2023-11-13

Publish Date

2024-04-01

Page Start

315

Page End

327

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 67_Issue 4_Pages 315-327.pdf

PDF . 4.5MB

Link

https://ejchem.journals.ekb.eg/article_330214.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=330214

Order

330,214

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Biochemical Identification of Some True Halophytes Using DNA Barcoding in the Egyptian Red Sea Coast

Details

Type

Article

Created At

30 Dec 2024