Novel metal complexes have been synthesized and characterized using elemental analysis, spectroscopy (1H NMR-FT-IR, mass, UV–visible) and molar conductance. The analytical data show that the metal to ligand ratio is 1:1. The antimicrobial activity of the ligand and their metal complexes were tested. The Schiff base-metal complexes Co(II), Ni(II), Cu(II) and Cd(II) are highly biological significance molecule for antimicrobial activity, as a result this study has been designed for investigation the synthesis, characterization, in vitro antibacterial and antifungal evaluation. For evaluation the In vitro study, the antibacterial activity has screened against six human pathogenic bacteria and two fungal strains. It was revealed that the prepared metal complexes illustrate higher antibacterial and antifungal activities than the metal free ligand. The highest antibacterial activity conveys against E. coli through disc diffusion method, and it is near to the standard (Ampicilin) Finally, the highest biological activity in views of both in vitro antibacterial and antifungal studies was proven to Co(II) complex.