Three polyextremophilic fungal isolates were isolated from agriculture soil at Mitsalsil, Dakahlia Governorate, Egypt. They tolerated temperature till 45 ºC and pH range from (1-14). They fully identified morphologically and genetically as Aspergillus niger; Aspergillus terreus HDJZ-ZWM and Paecilomyces variotii. The three fungal isolates secreted cellulase enzyme at neutral and acidic conditions also at different temperature degrees (28 ºC and 45 ºC). Lipase enzyme not secreted by Aspergillus terreus while secreted in neutral condition at 28 ºC and 45ºC by Aspergillus niger and at 45 ºC in acidic pH by Paecilomyces variotii. All fungal isolates secreted amylase at acidic pH at 45 ºC. Protease was secreted at all pH and temperatures used by Paecilomyces variotii, while secreted in neutral condition at 45 ºC by Aspergillus niger and at 28 ºC and 45 ºC by Aspergillus terreus.