Beta
304622

Chemotherapeutic Effect of Stigmasterol in Sorafenib Treated Breast Cancer Cell Lines via Modulation of NF-κB and ERK Signaling Pathways

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

-

Abstract

Abstract
Breast cancer is the main predisposing factor for female tumor-related death globally, which increases the requirement to investigate the effectiveness of new drug combination strategies. Sorafenib is a multi-kinase inhibitor mainly targeting vascular endothelial growth factor receptor (VEGFR) and Ras/Raf/ Extracellular signal-regulated kinase (ERK) pathway. Stigmasterol is a phytosterol with anticancer activity targeting different oncogenic pathways. This study aimed to examine the antitumor effects of stigmasterol and sorafenib combination against MDA-MB-231 and MCF-7 breast cancer cell lines via assessing their impact on VEGF, VEGFR-2, nuclear factor kappa B (NF-κB), Ki-67, Bcl-2, ERK, and caspase-3. Cytotoxicity was investigated using the MTT assay. VEGF, VEGFR-2, ERK, NF-κB, Bcl-2, and Ki-67 levels were assessed using the ELISA technique. VEGFR-2 gene expression was assessed using the RT-PCR technique, while caspase-3 activity was investigated using the colorimetric technique. Sorafenib and stigmasterol combination reduced the levels of NF-κB, Bcl-2, Ki-67, VEGFR-2, and VEGF-A, whereas the activity of caspase-3 was increased. In addition, MCF-7 showed more favorable results than MDA-MB-231. Stigmasterol and sorafenib combination may be a promising therapeutic regimen for breast cancer treatment through modulation of NF-κB-VEGF/BCL-2 and ERK/Caspase-3 signaling axes crosstalk.

DOI

10.21608/ejchem.2023.204388.7825

Keywords

breast cancer, Sorafenib, Stigmasterol, Angiogenesis, apoptosis

Authors

First Name

asmaa

Last Name

elshamy

MiddleName

-

Affiliation

Faculty of pharmacy damanhour university

Email

asmaa.elshamy@pharm.dmu.edu.eg

City

-

Orcid

-

First Name

Gamal

Last Name

Omran

MiddleName

-

Affiliation

Biochemistry Department, Faculty of Pharmacy, Damanhour University, Egypt, postal code: 22511

Email

gamal.omran@pharm.dmu.edu.eg

City

-

Orcid

-

First Name

Mohammad

Last Name

Abd-Alhaseeb

MiddleName

-

Affiliation

Pharmacology and Toxicology Department, Faculty of Pharmacy, Damanhour University, Egypt, postal code: 22511

Email

m.abdelhasseb@pharm.dmu.edu.eg

City

-

Orcid

-

First Name

MAHA

Last Name

HOUSSEN

MiddleName

-

Affiliation

Biochemistry department faculty of pharmacy Damanhour University

Email

mahahoussen@pharm.dmu.edu.eg

City

-

Orcid

-

Volume

67

Article Issue

3

Related Issue

45192

Issue Date

2024-03-01

Receive Date

2023-04-16

Publish Date

2024-03-01

Page Start

227

Page End

234

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_304622.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=304622

Order

304,622

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Chemotherapeutic Effect of Stigmasterol in Sorafenib Treated Breast Cancer Cell Lines via Modulation of NF-κB and ERK Signaling Pathways

Details

Type

Article

Created At

30 Dec 2024