Producing antibacterial textile using β cyclodextrin as a one agent emulsifier for thyme oil and reducing agent for silver nanoparticles were successfully prepared and characterized. Produced emulsion and AgNPs produced using cyclodextrin were examined using Polar microspore, TEM and XRD. Physical properties of Treated cotton fabrics properties as morphology were examined using SEM-EDS. Besides antimicrobial activity were also examined against most popular infectious bacteria (E-coli). Obtained results confirmed synthesis of thyme emulsion oil as microsphere between 5-8 μm using 1:4 (emulsifier: β cyclodextrin) observed under optical microscope. While TEM confirmed producing spherical AgNPs using β cyclodextrin with particle size 5-15 nm which confirmed by XRDs via characteristic peaks belongs to AgNPs. Incorporation binary thyme oil emulsions and AgNPs previously prepared by β cyclodextrin, showed deposition of emulsion appeared on the surface of fabrics as a coated film compared with blank cotton
fabric under SEM, while EDX indicated the prepsence of AgNPs in sample treated with thyme emulsion/ β-CD/AgNPs which proves the successful inclusion of AgNPs. Treated fabric using emulsion/ β-CD/AgNPs showed high antibacterial activity towards E. Coli and S. aureus that candidate produced fabrics treated thyme emulsion/ β-CD/AgNPs for antibacterial and medical applications.