Recently, nanotechnology plays an important role in solving environmental problems such as wastewater treatment. Metal oxides such as copper oxides and zinc oxides have a role in water purification. Hence, this work aimed to remove Methyl blue dye from a synthetic wastewater sample using an environmentally friendly and cost-effective bio-sorbents; copper\ zinc oxide bimetallic (CuO\ZnO) which was synthesized by a green method using Fussarium oxysporum extract, and the biosorption performance was evaluated through isothermal and kinetic studies. The bio-synthesized CuO\ZnO nanoparticles were characterized by UV–Vis spectrophotometry and transmission electron microscopy (TEM). From TEM micrographs, CuO\ZnO particle size ranged from 9-40 nm and UV spectrophotometry showed the characteristic peak at 241 nm. The bio-synthesized CuO\ZnO NPs had an antibacterial activity against (Staphylococcus aureus, Bacillus subtilis) which represent Gram-positive bacteria, and (Escherichia coli, Klebsiella sp) which represent Gram-negative bacteria was studied resulting in a maximum clear zone of 1mg\mL concentration against Escherichia coli and Staphylococcus aureus more than Klebsilla sp and Bacillus subtilis. The experimental data showed that the Langmuir model and the pseudo-second-order model were fitted to the data and the biosorption capacity reached a maximum and was recorded as 68.199 mg/g.