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310422

Bioremediation of heavy metals from synthetic water by endophytic bacteria isolated from floating hydrophytes

Article

Last updated: 01 Jan 2025

Subjects

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Tags

Material science

Abstract

One of the most pressing environmental issues worldwide today is heavy metal pollution. This study aimed at isolating endophytic bacteria from the internal cells of the roots, stems, and leaves of Lemna gibba and Eichhornia crassipes collected from a permanent pond in the Nile Delta, to remove various heavy metals (Cu, Cr, and Pb). Of the 44 total isolates, 34 isolates were considered positive for exopolysaccharides. The halotolerance test revealed that all the isolates tolerated NaCl stress up to 15%, and when the bacteria were stressed by growth in 20% and 25% NaCl, 21 and 17, respectively, were able to grow. The growth kinetics of the isolates were examined with Pb and Cr metal (150 and 300 mg L-1) independently. To estimate the Pb ion minimum inhibitory concentration, four Pb-tolerant bacterial isolates were chosen. The isolates were grown in LB medium with 100-500 mg L-1 Pb metal ions and measured at 600 nm at predetermined time intervals (8, 12, 24, 48, and 72 h). All bacterial stains survived 100–500 ppm lead concentrations. In this work, the effectiveness of a single isolate, 2Pe, in removing Pb was evaluated. At an ion concentration of 20 mgL-1, isolate (2Pe) removal efficiency was 21.65%. The endophytic isolate (2Pe) was described morphologically and biochemically, and its name was identified by 16S rRNA sequencing. In the end, humans, animals, plants, and everything else in the ecosystem are all put under stress by the accumulation of heavy metals in the environment. To ensure efficient and cost-effective procedures, it is necessary to have a thorough awareness of the process and the available options for remediation at each stage.

DOI

10.21608/ejchem.2023.215078.8081

Keywords

hydrophytes, Lemna, Eichhornia, Endophytic bacteria, Heavy metals, bioremediation

Authors

First Name

Nariman N.

Last Name

El-Sebaaye

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt

Email

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City

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Orcid

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First Name

Ashraf A.

Last Name

Elsayed

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt

Email

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City

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Orcid

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First Name

Yasser A.

Last Name

El-Amier

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

Volume

67

Article Issue

2

Related Issue

44642

Issue Date

2024-02-01

Receive Date

2023-06-02

Publish Date

2024-02-01

Page Start

437

Page End

451

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_310422.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=310422

Order

310,422

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Bioremediation of heavy metals from synthetic water by endophytic bacteria isolated from floating hydrophytes

Details

Type

Article

Created At

30 Dec 2024