Biological control is receiving increasing attention as an alternative means of disease control, especially where disease resistance or chemical control are not available. The aim of this study was to investigate the phytochemical analysis of emergent Cyperus laevigatus L and assess its antioxidant and antimicrobial activities. Methanol extract's preliminary phytochemical screening revealed differences in active secondary chemicals. For the rhizome, stem, and leaves, respectively, phenolics were found to be 267.06, 79.48, and 96.46 mg of gallic acid equivalent/g dry extract, flavonoids were 90.62, 27.78, and 34.47 mg of catechin equivalent/g dry extract; and tannins were 32.83, 12.46, and 23.35 mg of gallic acid equivalent/g dry extract. With an IC50 value of 30, 40, 42, and 51.76 mg/L for the rhizome, stem, and leaf, respectively, the DPPH antioxidant activity of the C. laevigatus extracted components showed that the rhizome extract is the most potent. The current findings indicate that C. laevigatus rhizome extract had superior antibacterial activity against the Gram-positive bacterial strains, while methanolic extract had a greater zone of inhibition against Pseudomonas aeruginosa and Escherichia coli (25.8 and 22.2 mm, respectively) (Staphylococcus aureus and Bacillus subtilis). The separation of pure components from the C. laevigatus extracts will be the focus of future research. Important biological results also suggested that more research on this plant may make it possible to find new medications made from natural sources.