Strontium oxide (SrO) bioglass was prepared with the sol-gel route, then exposed to gamma irradiation with different doses. In this work, Bioactivity was studied by in-vitro synthesis throughout immersion of samples in simulated body fluid (SBF) for different time intervals showing the effect of at different doses on the in vitro bioactivity and solubility was studied when SrO was replaced by biologically active CaO glass. Fourier transform infrared (FTIR) spectroscopy was used to evaluate the chemical structure of the studied samples before and after exposure, results indicated the formation of silica- and hydroxyapatite-rich layers. Increasing the substitution of SrO for CaO decreased the temperatures which resulted in a glass lattice which described as slightly stronger. In addition, the dissolution rate of the initial glass increased with the SrO content. The formation of hydroxyapatite (HAp) layers increased across the board, but the layer was thinner and contained strontium in the SrO-containing glass. The results showed also that replacing SrO with CaO in bioglass before and after gamma irradiation increased glass dissolution because of the generation of no-bridging oxygen (NBOs) across the glass network with gamma radiation, with this effect being more pronounced for Sr-containing glass. Correlating the results together, it is clear that, irradiation shows a remarkable effect on the bioactivity of the studied glass samples.