Liver diseases are emerging global health issues, non-alcoholic fatty liver disease (NAFLD) represents the most prevalent severe liver disease worldwide in the 21st century. Besides environmental factors, genetic predisposition triggers NAFLD development. This study investigated the sensitivity of Resistin (rs1862513) gene polymorphism in NAFLD and hepatic fibrosis in NAFLD Egyptian patients. The study was performed on 126 subjects as 63 healthy control subjects and 63 NAFLD patients. Gene polymorphism for NAFLD patients and healthy controls were done by PCR method. The biochemical parameters in NAFLD group showed a significantly elevated WBCs count; ALT; AST; TG; FBS; NFS; serum insulin; HOMA-IR; Total and direct bilirubin when compare with control and showed a significant decrease in both albumin level and PLT count. There was no significant differences regarding genotypic and allelic frequencies among NAFLD subjects and control individuals. Fibrosis progression estimated by NFS may accompanied with higher age. PLTs, WBCs count, Hb and TG significantly decreased while INR, AST, total and direct bilirubin, FBS and HOMA-IR increased in correlation to level of fibrosis. Genotype frequencies differed significantly among the different fibrosis stages groups. CG+GG genotype carriers showed significantly higher age and ALT while lower WBCs count with fibrosis progression. Our study stated that the best diagnostic tool was FBS followed by direct bilirubin that also proved as a good tool in detecting NAFLD patients with AUC 1.000 and 0.781, sensitivity of 100% and 60% for FBS and direct bilirubin respectively and 100% specificity for both. AST displayed the greatest AUC, sensitivity and also specificity values (1.000, 100%, 100% respectively) for detecting all G allele possessing patients.