Silver nanoparticles (AgNPs) were successfully synthesized via laser ablation technique in aqueous media. Polymer blends of Chitosan (CS), polyvinylpyrrolidone (PVP), and Amoxicillin (AMOX) were synthesized using a casting technique from a common solvent. Ag NPs were incorporated into CS/PVP/AMOX blend matrix to outline the role played by AgNPs on the blend's main characteristics. Synthesized nanoparticles show a surface plasmon resonance peak at about 430 nm. XRD pattern of the synthesized silver reveals the formation of homogenous multi-dispersed nanoparticles with an average size of 14 nm calculated using the Scherrer equation and with an agreement with that obtained from transmission electron microscopic (TEM) image. The optical energy gap is calculated from the electronic UV/Vis. transitions in combination with the measured electrical conductivity of the studied samples imply a composition dependence on the obtained values. The antibacterial effect of the samples was measured, which shows a correlation with the added amount of the dopant materials.