In Egypt, Culex pipiens (Diptera: Culicidae), has been investigated and declared as a vector of several human diseases. Here, safe, eco-friendly, and high-potency alternate insecticides were used. For this purpose, metal-organic frameworks (MOFs) with different metal centers (Iron, cobalt, manganese and nickel) were synthesized as they were used as anti-mosquitoes. MOFs with different concentrations to give mortality between 20 – 90% for the third larval instar were used. Also, sub-lethal concentration was investigated its effect on some biological activities (life cycle disorder, fecundity, sterility index, and egg-hatchability). The highest value of LC50 and LC95 were 110.55 & 389.48 ppm for Co-MOFs and the lowest value of LC50 and LC95 were 42.64 & 241.33 ppm for Fe-MOFs after 48hrs. The reproductive potency was affected as a result of the applied Fe-MOFs and Mn-MOFs. The damage that occurred from Fe-MOFs was more than that happened from Mn-MOFs; the sterility index was 100% and 75.2% for Fe-MOFs and Mn-MOFs respectively.