Biobased- polysaccharide polymers were synthesized using two different harsh environments the inhabitant (oil based medium and high salt medium) fungal isolates. They have shown 99.5% identity of 98% query to Aspergillus terreus and 99.37% identity of 99% query to Penicillium griseofulvum strain P-1707, respectively on genetic identification. They were given the name and code Aspergillus terreus, f1 and Penicillium griseofulvum, f2, respectively. Both isolates exhibited strong affinity of growth on different wastes with the highest biopolymers productivity (1.25 & 1.27 g/l) while using the sesame husk (Sem) as the starting raw material. Statistical optimization using Placket-Burman design increased biopolymers yield up to 2.74 & 2.13g/l, respectively at dipotassium hydrogen phosphate (K2HPO4), 2.77 g/l; magnesium sulphate heptahydrate (MgSO4.7H2O), 1.421 g/l; Yeast Extract, 3; Sesame husk, 57.3; Inoculum Size, 44.66% ; Initial pH 5, Incubation Temperature 50 OC and Incubation period 4.2 days, While f2 at K2HPO4, 3 g/l; MgSO4.7H2O, 2 g/l; Yeast Extract, 5; Sesame husk, 95.42 g/l; Inoculum Size, 1%; Initial pH 8, Incubation Temperature 37.5 OC and Incubation period 6 days. The biopolymers were purified using the ethanol precipitation method. The finally selected two biopolymers were characterized using appropriate techniques including FTIR, 1H NMR and viscosity measurements confirmed their polysaccharide nature. The purified polysaccharide biopolymers were applied on Herpes simplex type 1virus (HSV-1) cells at maximum non-cytotoxic conc. (MNCC) developed from cytotoxicity measurements giving a moderate antiviral activity with EC50 of 94.23±2.65 and157.98±3.52. These biopolymers represented a promising environmentally benign antiviral therapy against HSV-1.