Discharge textile wastewater without any treatment may have negative impact on the physio-chemical, microbiological features of domestic wastewater. Therefore, the aim of this study was to determine the physical-chemical, heavy-metals, toxicity, and microbiological characteristics of textile wastewater collected from five textile factories and surface water collected from River Nile, Ismailia canal and El-Rahawy drain water. The obtained results of physical-chemical parameters in textile wastewater influents showed that, pH ranged between 7.1 and 12.2. However, TDS ranged from 2348 to 7049.8 mg/L, TSS varied from 83.6 to 363 mg/L. Moreover, COD and BOD in influents of textile wastewater varied 319.7 and 902.7 mg/O2L and 108.2 to 367.5 mg/O2L, respectively. On the other hand, COD, BOD and oil in El-Rahawy drain water reached to 189.8, 77.8, and 9.3 mg/L, respectively. Bacterial indicator, some selected pathogenic bacteria and dye degrading bacteria (DDB) were detected in the majority of the collected samples. The highest values of total coliform (TC), fecal coliform (FC) and fecal streptococci (FS) in textile wastewater were reached to 6.05×105, 5.98×105 and 5.85×105MPN/100 mL, respectively. The tested heavy metals in both textile wastewater and environmental water samples were within the permissible limits of law 44/2000. All textile wastewater and environmental water samples were toxic except samples collected from Jeans Care Factory and River Nile water. The textile wastewater was not complying with the environmental legislation (Law 48/1982 Ministerial Degree 92/2013). Efficient treatment process for textile wastewater before mixing with municipal wastewater is highly needed.