Novel wound dressings were prepared by treating non-woven cotton fabric samples with different formulations of carbopol (CP), hyaluronic acid (HA), and sodium alginate (HA) followed by crosslinking with Ca2+, Zn2+ or Cu2+ ions. The impacts of such formulations on the antibacterial besides, some physico-mechanical properties of such dressings were studied. Loading the crosslinked CP/HA/SA dressing with sodium diclofenac and its in-vitro releasing from that dressing at pH 7 was investigated. To improve the antibacterial activities of such dressing, silver nano-particles were incorporated as a bioactive agent in the dressing formulation. The CP/HA/SA crosslinked film was characterized via FTIR analysis whereas the CP/HA/SA dressing SEM and EDX images were investigated.