Silver nanoparticles (Ag-NPs) are of great interest due to their wide use in many applications. In that research work, different types of pyrodextrins (PDs), namely British gum (BG), Dexy-84, and Dexy-86, were utilized as reducing and stabilizing agents for preparing Ag-NPs. The conditions affecting synthesis of Ag-NPs such as PD type, reaction temperature and time, reaction medium pH and AgNO3/PD molar ratio were studied. The results obtained exhibit that BG is the effective PD type to synthesis Ag-NPs. The optimum conditions of the synthesis reaction are: AgNO3/BG molar ratio, 0.075; pH, 11; reaction time, 3h; and reaction temperature, 70 OC. Cotton fabrics treated with different finishing formulations containing the prepared Ag-NPs exhibit antibacterial properties against both the E. coli and S. aurous bacteria.