Due to their capacity to impart certain qualities, such as increased strength and functionality, nanoparticles are being used as a treatment agent for cellulose acetate fibres. It was attempted to provide cellulose acetate (CA) fabric special qualities by treating it with titanium dioxide nanoparticles (TiO2 NPs) in various alcoholic solutions with or without pretreatment by excimer laser irradiation. We evaluated weight loss, area shrinkage, tensile strength, wettability, whiteness index, antibacterial activity, and UV protection factor to determine the impact of various treatments on the characteristics of acetate fabric (UPF). Scanning electron microscopy is used to study surface morphology (SEM). FT-IR and EDX are also provided. The outcomes show that nano TiO2 treatment in an alcoholic medium with or without laser pretreatments greatly improves wettability, UPF, and antibacterial activity. Additionally enhanced are the fiber's tensile strength and whiteness.