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Synthesis, characterization and anticancer potency of novel Mn(II), Co(II), and Cu(II) complexes based on N-[(1E)-1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)ethylidene]-2-(4-methy

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Inorganic chemistry

Abstract

Metal (II) complexes based on the ligand, N-[(1E)-1-(4-hydroy-6-methyl-2-oxo-2H-pyran-3-yl)ethylidene]-2-(4-methylanilino)acetohydrazide (HL) with Co (II), Mn (II), and Cu (II) were successfully synthesized and characterized by the use of analytical, spectral (IR, UV-vis, 1H NMR), and thermal (TGA) measurements. Also, magnetic moment, molar conductance, and electron spin resonance (ESR) spectral assignments for Cu(II) complexes were measured. Moreover, the newly synthesized complexes were tested against Huh-7, HCT-116, A549 cancer cell lines using MTT assay. Their apoptotic activity was determined through flow cytometry using Annexin-V-FITC/PI. Moreover, their ability to arrest cell cycle was studied. Results revealed that the complex of Mn (II) is a high spin octahedral, cobalt (II) complexes are high spin tetrahedral, and copper (II) complexes are square planar. Also, Mn (II), Co (II), and Cu (II) complexes of the ligand showed cytotoxicity activity. The tested complexes showed cytotoxic activity towards three human cancer cell lines, particularly complexes 3 and 5. Complex 3 recorded IC50 values 10.51 µM and 24.96 µM, respectively against Huh-7 and A549 cells. Complex 5 showed IC50 values 28.86 µM and 7.2 µM, respectively against Huh-7 and A549 cells. Furthermore, results of the Annexin-V-FITC/PI apoptosis assay revealed that compounds could induce apoptosis in cancer cells by increasing the percentage of apoptosis. Complex 3 induces total apoptosis of 37.15% in Huh-7 cells while complex 5 gives 34.15% in A549 cells. Complex 3 gives a significant diminishing of cells in G0/G1 phase in HCT-116 cells, complex 5 caused less accumulation in G0/G1 phase in A549 cells. In conclusion, the new complexes exerted their anticancer activity through induction of apoptosis and cell cycle arrest of cancer cells.

DOI

10.21608/ejchem.2023.196938.7652

Keywords

Dehydroacetic acid, hydrazone, metal (II) complexes, anticancer, apoptosis, Cell cycle analysis

Authors

First Name

Fathy A.

Last Name

El-Saied

MiddleName

-

Affiliation

Chemistry Department, Faulty of Science, Menoufia University, Shebin El-Kom, Egypt

Email

elsayedfathy139@yahoo.com

City

Shebin El-Kom

Orcid

-

First Name

Tarek A.

Last Name

Salem

MiddleName

-

Affiliation

Molecular Biology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt

Email

salem_tarek@yahoo.com

City

-

Orcid

-

First Name

Alaa

Last Name

Metwaly

MiddleName

N.

Affiliation

Chemistry Department, Faulty of Science, Menoufia University, Shebin El-Kom, Egypt

Email

alaa_nasr_228@yahoo.com

City

-

Orcid

-

First Name

Bishoy

Last Name

El-Aarag

MiddleName

-

Affiliation

Biochemistry Division, Chemistry Department, Faculty of Science, Menoufia University, Shebin El-Koom 32512, Egypt

Email

bishoy.yousef@gmail.com

City

-

Orcid

0000-0001-8520-347X

Volume

67

Article Issue

1

Related Issue

44392

Issue Date

2024-01-01

Receive Date

2023-02-28

Publish Date

2024-01-01

Page Start

37

Page End

55

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_308579.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=308579

Order

308,579

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Synthesis, characterization and anticancer potency of novel Mn(II), Co(II), and Cu(II) complexes based on N-[(1E)-1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)ethylidene]-2-(4-methy

Details

Type

Article

Created At

30 Dec 2024