The finding as well as the subsequent medical history of antimicrobials has become one of the most significant game modifications in medical history. As a result, a natural substance of 2-Methyl butyl propyl phthalate was extracted from a marine-derived Streptomyces parvulus strain MDA remoted originating along the Red Sea coast; Hurghada. The elimination of Streptomyces parvulus strain MDA via 2-Methyl butyl propyl phthalate became an exciting topic in our study; locating was tested using a minimal bactericidal concentration (MBC) of 300 µg/ml and an 80 µg/ml minimum inhibitory concentration (MIC). The kinetic time killer graph illustrated that the microorganisms damaged cells in 5 hours. The fractionation and purification of 2-Methyl butyl propyl phthalate were completed by the use of silica gel column G254, thin layer chromatography (TLC), liquid chromatography (LC), and nuclear magnetic resonance (NMR), which helped in the elucidation of the structure of 2-Methyl butyl propyl phthalate with the molecular components of C16H22O4.