Recently, developing of the biomedical materials to maintain optimum functionality has become extremely important. From that point of view, non-woven viscose (NWV) fabric based wound dressings containing Na-alginate (Alg) or its blends with poly aspartic acid (Pas), carboxymethyl cellulose (CMC) or carrageenan (CG), as an anionic layer, and chitosan (CS), as a cationic layer, were prepared via layer - by - layer technique. In addition, nano silver particles (Ag-NPs) were included during processing the last coating layer of the dressings. Factors influencing the building up of these dressings like Pas/Alg weight ratio, coating layers number and the anionic coating layer formulation were studied. The outcomes obtained revealed that: i) the ratio 50/50 of Pas/Alg is the proper ratio to attain high swellability along with remarkable antibacterial activities, ii) increasing of coating layers leads to an improvement in swellability, gel fraction, stiffness, and antibacterial activities in addition to a decreasing in air permeability of treated fabric, and iii) replacing the Pas by CMC or CG in building up of such dressings gave rise to arranging the aforementioned characteristics as: the gel fraction (CG/Alg-CS > Pas/Alg-CS > CMC/Alg-CS), the stiffness (Pas/Alg-CS > CG/Alg-CS > CMC/Alg-CS), the air permeability (CMC/Alg-CS > Pas/Alg-CS > CG/Alg-CS), and the antibacterial activity (Pas/Alg-CS > CG/Alg-CS > CMC/Alg-CS). Among the aforementioned prepared dressings, the Pas/Alg-CS dressing were characterized via SEM and EDX analysis which clearly showed the dressing morphology and confirmed loading of that dressing with the nominated bio-polymers and Ag-NPs.