Chitosan (CS) and acrylic acid were combined in a Michael addition process to successfully produce N-carboxyethylchitosan (CECS). Then carboxyethyl chitosan undergo ionic gelation method to prepare carboxyethylchitosan nanoparticles (CECSNPs). The prepared Chemical material replacement is appropriate for modern chemical processes and ecofriendly. Cotton, viscose, and polyester/cotton blend fabrics were all treated with nano carboxyethylchitosan at varying concentrations. 0.5, 1, 1.5 wt%, then dyed with commercial acid dyes. We also tested the dyed fabrics' antimicrobial resistance to two model gram-negative and gram-positive bacteria. Further, The K/S, light, rubbing, prespiration, and washing fastness values were estimated. This paper aims to treatment Cotton, viscose, and polyester/cotton blend fabrics with different concentration of nano carboxyethylchitosan and apply two commercial acid dyes on it. These dyes are used in dyeing these treated fabrics without any additives and all treated fabrics have good K/S, light fastness, and are also excellent for washing, rubbing and prespiration fastness. Also, they have highly antimicrobial activity.Use of nanocarboxyethylchitosan to treatment of many fabrics before dyeing will bring a number of benefits to society including higher fastness properties without any additives thus they save a lot of money, materials and have ecofriendly to environment