Akermanite-based glass ceramics were prepared with /without Ag2O. Different techniques (DTA, XRD, SEM, and FTIR) are used for the characterization of the synthesized samples. For all prepared glass-ceramic samples, bioactivity, degradation, and ion release were tested in simulated body fluid (SBF). Also, the antibacterial effect was tested with Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Moreover, the cytotoxicity against the normal fibroblast (Wi-38) cell line was studied. DTA shows the addition of Ag2O lowered the glass transition temperatures to a little extent. the XRD results show that raising the sintering temperature from 900 ˚C to 1000 ˚C led to more crystallinity of Akermanite, cuspidine, and monticellite phases. The microstructure shows submicron crystals in a glassy matrix. After immersion in SBF, nano- and submicron particles of hydroxyapatite were detected in all samples. The integration of silver in the samples enhanced their ability to form hydroxyapatite and increased their antibacterial activity. Cytotoxicity test indicated that all samples up to concentration 125 um/ml had no toxic effect on cells and could be safely used as biocompatible materials.