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295033

Phytochemical Analysis and Antimicrobial Activity of Euphorbia milii

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Organic chemistry

Abstract

Antimicrobial activities of Euphorbia milii (Euphorbiaceae) extract and its sub-fractions against Gram-negative, Gram-positive bacteria, unicellular yeast and two filamentous fungi were evaluated and the minimum inhibitory concentrations were determined. The ethyl acetate fraction (EAf) exhibited the most significant antimicrobial activity against five tested pathogenic microbes. For the first time, liquid chromatography-electrospray ionization-time-of-flight mass spectrometry analysis (LC-ESI-TOF-MS) of EAf was used to identify its chemical composition. LC-MS/MS analysis allowed the tentative identification of 22 compounds. Whereas quercitrin, luteolin, quercetin, kaempferol-3-glucuronide and naringenin were the major constituents. The chemical composition of unsaponified and saponified from n-hexane fraction was analysed by gas chromatography/mass spectrometry (GC-MS). The oxygenated compounds represent the major constituent in both fractions (56.48 and 61.97%, respectively). Terpenes represented by 30.94% in unsaponified fraction while fatty acids and esters constituted by 18.91% in the saponified fraction. Quantification of the total phenolics and flavonoids of the methanolic extract and its successive fractions were determined. The highest percent of the total phenolic was observed for methanolic extract while total flavonoids were recorded for n-butanol and ethyl acetate.

DOI

10.21608/ejchem.2023.192476.7575

Keywords

LC-MS/MS, GC-MS, Euphorbiaceae, Euphorbia milii, antimicrobial activity, flavonoids, phenolics

Authors

First Name

Amal Z.

Last Name

Hassan

MiddleName

-

Affiliation

Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Institute, National Research Center, El‑Behoos st, Dokki, Giza 12622, Egypt

Email

-

City

-

Orcid

-

First Name

Heba-tollah M.

Last Name

Sweelam

MiddleName

-

Affiliation

Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Institute, National Research Center, El‑Behoos st, Dokki, Giza 12622, Egypt

Email

-

City

-

Orcid

-

First Name

Kamel H.

Last Name

Shaker

MiddleName

-

Affiliation

Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Institute, National Research Center, El‑Behoos st, Dokki, Giza 12622, Egypt

Email

kamelshaker11@yahoo.com

City

6 October

Orcid

0000-0001-9876-1251

First Name

Moustafa M.

Last Name

Zohair

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Institute, National Research Center, El-Behoosst., Dokki-Cairo 12622, Egypt , Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819‑0395, Japan

Email

-

City

-

Orcid

-

First Name

Warda E.

Last Name

Ashour

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Institute, National Research Center, El-Behoosst., Dokki-Cairo 12622, Egypt

Email

-

City

-

Orcid

-

First Name

Howaida I.

Last Name

Abd-Alla

MiddleName

-

Affiliation

Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Institute, National Research Center, El‑Behoos st, Dokki, Giza 12622, Egypt

Email

-

City

-

Orcid

-

Volume

66

Article Issue

13

Related Issue

43707

Issue Date

2023-12-01

Receive Date

2023-02-13

Publish Date

2023-12-01

Page Start

461

Page End

473

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 13_Pages 461-473.pdf

PDF . 984.5kB

Link

https://ejchem.journals.ekb.eg/article_295033.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=295033

Order

295,033

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Phytochemical Analysis and Antimicrobial Activity of Euphorbia milii

Details

Type

Article

Created At

30 Dec 2024