Previous studies have utilized different techniques to examine the presence of alpha-fetoprotein (AFP) receptors in tumour tissues. However, none of these studies has investigated the cytosolic and nuclear AFP receptors in gastric adenocarcinoma and gastric lymphoma tissues using radioreceptor assay studies. To address this gap, a radioreceptor assay was developed to determine the AFP receptor concentrations in homogenates of these tissues and optimize the conditions of labelled AFP binding with its cytosolic and nuclear receptors, which include pH, temperature, time of binding, and 125I-AFP concentration. The study revealed the presence of AFP receptors in gastric adenocarcinoma more than those in gastric lymphoma tissues and indicated that the binding efficiency of AFP receptors with the 125I-AFP in nuclear fractions was higher than those in cytosolic fractions for both tissue types. The developed radioreceptor assay represents a novel approach for the detection and quantification of cytosolic and nuclear AFP receptors in these types of tissues and could be recommended for assessing AFP receptor concentrations in gastric cancer tissues as a method for early cancer detection. This technique might aid the design of a novel cancer detection and treatment approach using AFP receptor-based nanoparticles and reveal novel mechanisms of AFP-receptor antitumour immune responses in the future.