The most prevalent neurological condition in children is febrile seizures (FS), which affects humans. It is possible to explore the underlying processes of FS that are connected to the onset of epilepsy and associated comorbidities using models of hyperthermia for seizure induction. The current study concentrates on examining the changes in the expression of a gap junction protein in two brain areas; the hippocampus and the cortex in young rats who experienced FS. Animals used in experiments were split into two groups, the cage control group, and the hyperthermia-stimulated FS group. Each group of the two groups was further subdivided into young (Y) and Adult (A) groups. Reduction in the expression of Cx43 has been detected in both groups of animals (Y & A) and was suggested to be related to seizure activity. The present study sheds more light on the possible role played by gap junction proteins in FS and epilepsy.