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297560

In vitro anticancer and antimicrobial activities of copper-zinc superoxide dismutase purified from Cellana rota snail

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Biochemistry

Abstract

The antioxidant, antimicrobial and anticancer activities of molluscan gastropods were elucidated in numerous previous studies. One of the most important antioxidant enzymes is superoxide dismutase (SOD) was homogeneously purified and characterized from Cellana rota snail crude extract. The purification of C. rota SOD was carried out through chromatographical separation on anion exchanger and size-exclusion resins. The specific activity of the purified SOD was 520.7 units mg-1 protein, representing 6.6 folds over the crude extract. The purified SOD was found 180-kDa monomeric as detected by gel filtration and SDS PAGE. It was greatly enhanced with CuCl2 and ZnCl2 and strongly inhibited with KCN and H2O2, proposing it as a Cu/Zn-SOD enzyme that termed Cellana rota copper-zinc superoxide dismutase (CrCu/Zn-SOD). The antimicrobial screening of CrCu/Zn-SOD indicated its antibacterial capacity against E. coli, S. typhi and S. aureus with MIC value of 0.78 µg/mL for the three pathogens. Moreover, it showed a MIC value of 1.56 µg/mL against the fungus A. niger compared to 5 µg/mL nystatin. CrCu/Zn-SOD showed potent in vitro toxicity towards adenocarcinomic human alveolar basal epithelial cells A549, human colorectal carcinoma Caco2 and human hepatocellular carcinoma HepG2 cancer cells with 2.451, 3.933 and 10.5 μg/mL IC50 values respectively. These preceding results suggest that CrCu/Zn-SOD from C. rota snail may have the potential to serve as a substrate for future antimicrobial and anticancer drug development.

DOI

10.21608/ejchem.2023.200467.7736

Keywords

Cellana rota, superoxide dismutase, Purification, Characterization, Antimicrobial, Anticancer

Authors

First Name

Mohamed

Last Name

Habib

MiddleName

R.

Affiliation

Medical Malacology Dept., Theodor Bilharz Research Institute, 1 Corniche El Nile St., Warrak El-Haddar, Imbaba, Giza 12411, Egypt

Email

m.habib@tbri.gov.eg

City

Giza

Orcid

-

First Name

Hassan M.

Last Name

Masoud

MiddleName

-

Affiliation

Molecular Biology Dept., National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt, Proteome Research Lab., Central Laboratories Network and Centers of Excellence, National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt

Email

hssnmasoud@yahoo.com

City

Giza

Orcid

-

First Name

Mohamed

Last Name

Helmy

MiddleName

S.

Affiliation

Molecular Biology Dept., National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt, Proteome Research Lab., Central Laboratories Network and Centers of Excellence, National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt

Email

mm.salah-eldin@nrc.sci.eg

City

Giza

Orcid

-

First Name

Ahmed A.

Last Name

Hamed

MiddleName

-

Affiliation

Microbial Chemistry Dept., National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt

Email

ahmedshalbio@gmail.com

City

Dokki

Orcid

0000-0002-9997-9284

First Name

Rasha M.

Last Name

Gad El-Karim

MiddleName

-

Affiliation

Medical Malacology Dept., Theodor Bilharz Research Institute, 1 Corniche El Nile St., Warrak El-Haddar, Imbaba, Giza 12411, Egypt

Email

dr_rashagad2008@yahoo.com

City

Giza

Orcid

-

First Name

Rasha

Last Name

Ali

MiddleName

E.M.

Affiliation

Medical Malacology Dept., Theodor Bilharz Research Institute, 1 Corniche El Nile St., Warrak El-Haddar, Imbaba, Giza 12411, Egypt

Email

roshaezzat@yahoo.com

City

Giza

Orcid

-

First Name

Khaled M.

Last Name

Zayed

MiddleName

-

Affiliation

Medical Malacology Dept., Theodor Bilharz Research Institute, 1 Corniche El Nile St., Warrak El-Haddar, Imbaba, Giza 12411, Egypt

Email

khaled.z111@yahoo.com

City

Giza

Orcid

-

First Name

Mahmoud A.

Last Name

Ibrahim

MiddleName

-

Affiliation

Molecular Biology Dept., National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt, Proteome Research Lab., Central Laboratories Network and Centers of Excellence, National Research Center, 33 El-Buhouth St., Dokki, Giza 12622, Egypt

Email

ma.ibrahim@nrc.sci.eg

City

Giza

Orcid

-

Volume

66

Article Issue

12

Related Issue

43517

Issue Date

2023-12-01

Receive Date

2023-03-16

Publish Date

2023-12-01

Page Start

1

Page End

10

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 12_Pages 1-10.pdf

PDF . 533.1kB

Link

https://ejchem.journals.ekb.eg/article_297560.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=297560

Order

297,560

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

In vitro anticancer and antimicrobial activities of copper-zinc superoxide dismutase purified from Cellana rota snail

Details

Type

Article

Created At

30 Dec 2024