Amenorrhea is the absence or abnormal cessation of menses. Primary and secondary amenorrhea describes the occurrence of amenorrhea before and after menarche, respectively. We aimed to investigate the hormonal changes and FSHR gene polymorphism in teenager females with primary amenorrhea. For this purpose, 100 adult women (age 25 ± 10 years) were examined for the appearance of the signs of puberty and menstruation for detecting the cases of primary amenorrhea. The women were divided into two main groups, normal women and amenorrhea. Gonadotrophic hormones, overian hormones, anti-mullerian hormone (AMH), oxidative/ antioxidant redox and Follicular stimulating hormone receptor (FSHR) were tested in all groups. Our results showed that women with amenorrhea suffered from high FSH, LH and prolactin compared to normal women. In addition, a significant reduction of ovarian hormones was detected in women with amenorrhea compared to normal women with a concurrent alteration of antioxidant/oxidative redox. A significant reduction of antimullerian hormone (AMH) and FSH receptors (FSHR) also recorded in amenorheastic women. We can concluded that amenorrhea is associated with sever hormonal dys-regulation, AMH could be used as a significant biomarker in diagnosis of amenorrhea and FSHR plays a pivotal role in the development of amenorrhea.