Beta
ManageSearchChatDiscover
312115

Phenazine-Producing Pseudomonas aeruginosa OQ158909: A Promising Candidate for Biological Activity and Therapeutic Applications

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Pharmaceutical Chemistry

Abstract

Natural phenazines, as secondary metabolites of microorganisms, have recently gained much attention for their potential application as biologically active antimicrobial compounds. The present study was performed in order to investigate the production of a biologically active phenazine compound by a locally isolated strain of Pseudomonas aeruginosa. Thirty different strains were isolated from soil and water on a selective medium. From them, isolate 14-WANRC, was selected due to its high productivity of colored secondary metabolites after 5 days of incubation in King-A broth medium. The isolate was further identified using some morphological, biochemical and physiological tests as well as using 16S-rDNA which revealed a 99% similarity with Pseudomonas aeruginosa and was therefore registered in the Gene bank under the accession number of OQ158909. The production ability of the studied strain was then improved by studying the effect of different physiological and growth conditions. The results showed that the maximum output of phenazine compounds was obtained when the isolated strain was cultivated in mineral salt medium (MS), under shaking conditions of 100 rpm, for five days at 35°C. The secondary metabolites produced were finally extracted using ethyl acetate and subjected to TLC for compounds separation. The selected band exhibited potent antimicrobial, antioxidant and anticancer activities.

DOI

10.21608/ejchem.2023.214109.8045

Keywords

Pseudomonas aeruginosa, phenazines, Biologically active compounds, secondary metabolites, microbial natural products, isolation and bacterial identification

Authors

First Name

Hossam

Last Name

El-Masry

MiddleName

Mohammed

Affiliation

Chemistry of Natural and microbial products department Pharmaceutical and drug industries research institute National research Centre

Email

drhossamnrc33@gmail.com

City

Giza

Orcid

0000-0002-5473-8081

First Name

Nagwa

Last Name

Atwa

MiddleName

Amin

Affiliation

Chemistry of Natural and Microbial Products Department, National Research Centre, Cairo, Egypt

Email

nagwaatwa@yahoo.com

City

-

Orcid

0000-0003-2717-8813

First Name

Ahmed

Last Name

El-Beih

MiddleName

-

Affiliation

Chemistry of natural and microbial products department, Pharmaceutical and drug industries institute, National Research Centre, Cairo 12622, Egypt.

Email

aae2eg@yahoo.com

City

-

Orcid

0000-0002-6960-027X

First Name

mona

Last Name

agwa

MiddleName

-

Affiliation

Chemistry of natural and microbial products department, Pharmaceutical and drug industries institute, National Research Centre, Cairo 12622, Egypt.

Email

magwa79@gmail.com

City

-

Orcid

0000-0003-0232-7616

First Name

Ishrak

Last Name

Khafagi

MiddleName

K.

Affiliation

Botany & Microbiology department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt

Email

ishrakkhafagi@yahoo.com

City

-

Orcid

-

First Name

samira

Last Name

mansour

MiddleName

resik

Affiliation

Botany & Microbiology department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt

Email

samirarmansour@hotmail.com

City

ismailia

Orcid

-

First Name

Ahmed

Last Name

Eldiwany

MiddleName

I

Affiliation

Chemistry of natural and microbial products department, Pharmaceutical and drug industries institute, National Research Centre, Cairo 12622, Egypt.

Email

ahmed_eldiwany@yahoo.com

City

cairo

Orcid

0000-0003-3917-1141

Volume

66

Article Issue

11

Related Issue

43443

Issue Date

2023-11-01

Receive Date

2023-05-29

Publish Date

2023-11-01

Page Start

281

Page End

303

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 11_Pages 281-303.pdf

PDF . 1.7MB

Link

https://ejchem.journals.ekb.eg/article_312115.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=312115

Order

312,115

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Phenazine-Producing Pseudomonas aeruginosa OQ158909: A Promising Candidate for Biological Activity and Therapeutic Applications

Details

Type

Article

Created At

30 Dec 2024