Type 2 diabetes mellitus (T2DM) could be important in the early diagnosis of menstrual abnormalities and female infertility (FI). Here, we assess the association between T2DM-associated metabolic disturbances and FI. This study included 300 female subjects. They were divided into five groups; each group was made up of 60 individuals. Group (1) Healthy fertile women as a control. Group (2) Non-obese fertile women with T2DM. Group (3) Obese fertile women with T2DM. Group (4) Non-obese infertile women with T2DM. Group (5) Obese infertile women with T2DM. Each subject donated 6 ml fasting blood samples; 2 ml was used for DNA extraction and genotyping analysis and the remaining 4 ml was used for collecting serum which was used to estimate total cholesterol (TC), triacylglycerols (TAGs), insulin, luteinizing hormone (LH), follicle stimulating hormone (FSH), dehydroepiandrosterone sulfate (DHEA-S), estradiol, progesterone, testosterone, and leptin levels. Present data showed significant high levels of LH, estradiol, testosterone, leptin, and BMI in obese T2DM (fertile or infertile) compared to non-obese T2DM (fertile or infertile), and in obese T2DM infertile female than obese T2DM fertile female. On the other hand, current results showed significant low levels of FSH, progesterone and DHEA-S levels in obese T2DM (fertile or infertile) compared to their counter parts in non-obese T2DM (fertile or infertile), and in obese T2DM infertile female than obese T2DM fertile one. In the T2DM population, the XbaI and PvuII polymorphisms in estrogen receptors-alpha (ESRα) gene were shown to be associated with risk of FI. The FI is reported here to be associated with obesity and T2DM complications. Thanks to the fasting hyperinsulinemia, hyperleptinemia, and imbalance of reproductive hormones, the fertility of diabetic women may be reduced.