Hepatotoxicity is an imperative drawback of treatment with antineoplastic Cisplatin (Cis). Gum Arabic (GA) is a drained Gummy dislodged from the Acacia Senegal branches and stems, composed of calcium, potassium, and magnesium salts of polysaccharide GA acid. The objective of the current study is to evaluate the capability of GA to keep liver save safe from damage caused by a single intraperitoneal weekly dose of 7.5 mg Cis/kg rats. Twenty-four albino rats were located randomly in 4 groups; G1 act as control, G2 received GA, G3 received Cis, and G4 received simultaneously both Cis and GA. The results showed that Cisplatin-induced elevation in liver enzymes and caused different histological lesions in the hepatic structures. Immunohistochemically moderate positive reactions for PCNA and Caspase-3 were detected in hepatocytes of Cis in comparison with control. While, GA as a protective antioxidant exhibited a significant improvement in biochemical, histological, and immunohistochemical parameters, suggesting the use of GA in combination with Cis to attenuate liver damage in cancer patients.