Agro-industrial wastes have environmental negative effects leading to climate changes if improperly managed. This study aimed to utilization of wheat germ as one of the most generated agro-industrial wastes to produce two valuable products and investigation of their potential application as wound healing agents. Wheat germ oil (WGO) was firstly obtained by cold pressing for the wheat germ, then poly unsaturated fatty acids (PUFAs) were produced through microbial conversion of remaining wheat germ oil cake using locally isolated Saccharomyces cerevisiae. Fatty acids composition of the extracted WGO and produced PUFAs were assessed by GC/MS. Finally, their impact as wound healing agents against Human Skin Fibroblast cell line (HSF) and antimicrobial effect on two common wound infectious bacteria were carried out. The extracted WGO was 14.6% (w/w) from the wheat germ while, the yield of produced PUFAs was 10.13 g/100 g substrate. Chemical analysis of the wheat germ oil cake revealed its high contents of protein and carbohydrates (38.57 and 39.17%, respectively). However, fatty acids composition showed that both WGO and PUFAs contained 64.07 and 61.43 % poly unsaturated fatty acids, respectively. Results indicated that PUFAs were more efficient that WGO in the ability to enhance wound healing of HSF at the first 24h. and the growth inhibition of both Pseudomonas aeruginosa and Staphylococcus aureus by 15 and 25%, respectively.