Beta
ManageSearchChatDiscover
280134

TLR2 Gene Polymorphism and MicroRNA 301 gene Expression Level as a Signature for Hepatocellular Carcinoma in Egyptian Patients

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

HCC is the most prevalent gastrointestinal malignant tumour worldwide. Genetic determinants of HCC occurrence have been always an interest for most hepatologists. The aim of our research to discover link between TLR2 gene (rs3804099) polymorphism, MicroRNA 301 gene expression and HCC incidence in an Egyptian cohort. Methodology: 247 participants were divided to 3 groups. Group 1: 85 adult HCC patients on top of HCV. Group 2: 82 chronic HCV patients. They were followed up for one year. 80 supposedly healthy subjects who were matched in age and sex with patients (Group 3). All participants were subjected to clinical evaluation and history-taking, laboratory investigations and molecular testing (TLR2 SNP analysis and microRNA 301 gene expression by qPCR). Results: Our study found that HCC patients were mainly males (60%), aged 53.67 ± 7.62years. TLR2 (rs3804099) CC genotype was significantly prominent in HCC patients (37,6%), CT prevails in hepatitis C patients (47,8%), while TT was the main genotype of healthy controls. Individuals carrying genotype TT were significantly associated with decreased risk of HCC (OR 1, 95% CI, P = 0.0001), while those of CC showed a higher HCC risk (OR 3.048 95% CI (1.269 – 7.320), P = 0.013). A highly significant variation in MicroRNA301 level regarding Barcelona B, D stages (P <0.001) and TNM classification with increased serum level in advanced stages, increased tumour size mainly with CC genotype [T3=27 (84.4%) T4 =5 (15.6%), lymph nodes (N2) and metastasis (M1)]. We concluded that TLR2 SNP CC variant carries the highest risk of HCC development, while microRNA301 expression can be considered diagnostic and prognostic marker in HCC Egyptian patients.

DOI

10.21608/ejchem.2023.183053.7379

Keywords

Hepatocellular carcinoma, Hepatitis C virus, MicroRNA301, TLR2 SNP

Authors

First Name

Samar

Last Name

E. Ghanem

MiddleName

-

Affiliation

Department of Clinical Biochemistry and Molecular Diagnostics, National Liver Institute, Menoufia University, Shebeen El‑Koum, Menoufia Egypt

Email

samarghanm77@yahoo.com

City

-

Orcid

-

First Name

Maha

Last Name

M. Elsabaawy

MiddleName

-

Affiliation

Depatment of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El‑Koum, Menoufia Egypt

Email

maha.ahmed@liver.menofia.edu.eg

City

-

Orcid

-

First Name

Mervat

Last Name

M. Abdelkareem

MiddleName

-

Affiliation

Depatment of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El‑Koum, Menoufia Egypt

Email

dr.mervat82@yahoo.com

City

-

Orcid

-

First Name

Marwa

Last Name

L. Helal

MiddleName

-

Affiliation

Department of Clinical Biochemistry and Molecular Diagnostics, National Liver Institute, Menoufia University, Shebeen El‑Koum, Menoufia Egypt

Email

marwa.helal@liver.menofia.edu.eg

City

-

Orcid

https://orcid.org/00

First Name

Warda

Last Name

Othman

MiddleName

-

Affiliation

Depatment of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebeen El‑Koum, Menoufia Egypt

Email

dr.warda82@yahoo.com

City

-

Orcid

-

First Name

Ghada

Last Name

M. Salah Eldin

MiddleName

-

Affiliation

Department of physiology, Medical college, Alazhr University for girls, Egypt

Email

ghadasalah5150@yahoo.com

City

-

Orcid

-

First Name

Shymaa

Last Name

A. Elaskary

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Shebeen El‑Koum, Egypt

Email

dr.shaimaaelaskary@yahoo.com

City

-

Orcid

-

First Name

Fathia

Last Name

I. EL‑Bassal

MiddleName

-

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Menoufia University, Shebin El‑Kom, Menoufia, Egypt

Email

drfathiabassal@yahoo.com

City

-

Orcid

-

First Name

Ashraf

Last Name

El Fert

MiddleName

-

Affiliation

Department of Clinical Biochemistry and Molecular Diagnostics, National Liver Institute, Menoufia University, Shebeen El‑Koum, Menoufia Egypt

Email

a.alfert@yahoo.com

City

-

Orcid

-

Volume

66

Article Issue

9

Related Issue

43167

Issue Date

2023-09-01

Receive Date

2022-12-26

Publish Date

2023-09-01

Page Start

479

Page End

490

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 9_Pages 479-490.pdf

PDF . 1.4MB

Link

https://ejchem.journals.ekb.eg/article_280134.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=280134

Order

280,134

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

TLR2 Gene Polymorphism and MicroRNA 301 gene Expression Level as a Signature for Hepatocellular Carcinoma in Egyptian Patients

Details

Type

Article

Created At

30 Dec 2024