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Optimization and Scaling-up of Cyclodextrin Glucosyltransferase Production by the Local Isolate <i>Bacillus paramycoides</i> MZ005634

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Last updated: 01 Jan 2025

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Abstract

Cyclodextrin glycosyltransferase is an important industrial enzyme that converts starch in to cyclodextrins (α-, β-, and ɣ-CDs). In this study our aim was to optimize the activity of produced CGTase for the production of β-CD from a local bacterial isolate and up-scaling the fermentation process. A terrestrial Bacillus paramycoides was isolated from soil obtained from sewage of Ismailia canal and identified by 16S rRNA analysis for the production of CGTase enzyme. Fractional factorial design (FFD) was applied to analyze the influence and describe the relationship between the tested variables: soluble starch, fermentation time, peptone, KH2PO4, inoculum size and pH. The results represent an improvement in CGTase activity of 1.10 folds when compared to that previously obtained using the basal medium under the same cultivation conditions. The generated model was found to be very adequate for CGTase activity (81.57% accuracy) as the experimental value was compared to the predicted value. Upon applying a series of batch fermentations with different agitation rate an increase in CGTase activity about 1.6 folds compared to that obtained using shaked flasks.

DOI

10.21608/ejchem.2023.176162.7219

Keywords

Beta-cyclodextrin, fractional factorial design (FFD), fermenter

Authors

First Name

AM

Last Name

Saleh

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, 12622 Giza, Egypt

Email

alaasaleh011@gmail.com

City

-

Orcid

0000-0002-9076-3102

First Name

RA

Last Name

Zaki

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, 12622 Giza, Egypt

Email

raniaalh@yahoo.com

City

-

Orcid

-

First Name

SK

Last Name

Gomaa

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, 12622 Giza, Egypt

Email

dr.sanaaibrahime@gmail.com

City

-

Orcid

-

First Name

HA

Last Name

Elrefai

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, 12622 Giza, Egypt

Email

dr.heba_ar@yahoo.com

City

-

Orcid

-

First Name

MS

Last Name

Shafei

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, 12622 Giza, Egypt

Email

mona__sayed@hotmail.com

City

-

Orcid

-

First Name

IS

Last Name

Abd-Elsalam

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, 12622 Giza, Egypt

Email

ibrahimabdelsalam69@gmail.com

City

-

Orcid

-

First Name

AH

Last Name

Elrefaey

MiddleName

-

Affiliation

Department of Industrial Engineering, College of Engineering, American University of Sharjah, United Arab Emirates.

Email

formalmoni@gmail.com

City

-

Orcid

-

Volume

66

Article Issue

9

Related Issue

43167

Issue Date

2023-09-01

Receive Date

2022-11-22

Publish Date

2023-09-01

Page Start

343

Page End

351

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 9_Pages 343-351.pdf

PDF . 1.2MB

Link

https://ejchem.journals.ekb.eg/article_278938.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=278938

Order

278,938

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Optimization and Scaling-up of Cyclodextrin Glucosyltransferase Production by the Local Isolate <i>Bacillus paramycoides</i> MZ005634

Details

Type

Article

Created At

30 Dec 2024