The aim of this study to examine the effect of some biostimulants on growth characters of paulownia seedlings under irrigation with saline water. Different concentrations of biostimulants (Mycrrohiza and Yeast at 5 and 10 gm ) were applied under the conditions of irrigation with salt water at four levels of water salinity (0,1000,2000 and 3000 ppm) on growth characters, photosynthesis pigments, carbohydrates , Proline, total indol , total Phenol and some Macro elements.The results indicated that application of Mycrrohiza and yeast at 10 gm/ pot combined with untreated salinity irrigation significantly increased plant height, stem diameter, fresh and dry weight of leaves and stems, compared with salinity at 3000 ppm plus untreated biostimulants treatment. While, irrigation at Zero salinity combined with Mycrrohiza at 10gm /pot followed by 5gm/pot and yeast at 10gm/pot significantly increased root length, root diameter, fresh and dry weight of roots, compared with salinity at 3000 ppm plus untreated biostimulants treatments. Mycrrohiza application (10gm/pot) was more effective on pigments content, Nitrogen and Potassium% under irrigation with tap water. The application of Yeast at 10gm/pot under non saline water resulted in increases the total carbohydrates. The interaction between salinity at 3000 ppm and untreated biostimulants significantly increased proline content, total phenol and sodium %. The application of yeast at 10gm/pot combined with untreated biostimulants significantly increased total indol. Application of Yeast at 10gm/pot and 5gm/pot combined with tap water gave the highest values of Phosphorus ( P%) and Magnesium (Mg %). The results indicated that irrigation the seedlings with untreated saline water followed by 1000ppm significantly increased the growth parameter and chemical constituents except proline content, total phenols, sodium, calcium, which increased with irrigation at 3000 ppm salinity.