In this study, there are117 cases divided into 93 cases of obese patients and 24 healthy cases that collected from AL Salam Teaching Hospital in Mosul city. An estimation of some biochemical parameters such as Visfatin hormone, total cholesterol, triglycerides, high density lipoprotein (HDL), very low density lipoprotein (VLDL), low density lipoprotein (LDL), potassium, chloride, lactate dehydrogenase (LDH) and Aspartate Amino Transferase (AST), body mass index (BMI) in serum of all cases, obese patients group are divided into four groups. They are, Obesity without disease (n=64), Obesity with Blood pressure (n=19), Obesity with Diabetes mellitus (n=10). The results showed a significant increase in the levels of visfatin hormone, BMI, TotalCholesterol, Triglycerides,VLDL -C, LDL –C, Chloride, AST activity, LDH activity and a significant decrease in HDL-C, potassium levels in obese, obese with blood pressure, obese with diabetic mellitus patients in comparison with healthy group , and in female in comparison with male. The results also showed appositive correlation between visfatin hormone and (Total Cholesterol, Triglycerides, VLDL-C, LDL-C , BMI, AST, LDH, Chloride) and a negative correlation between visfatin and ( Potassium, HDL-C).