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272029

Exploring The Inhibitory Potential of Massoia Oil on Biofilm Dual-Species Culture Of Staphylococcus aureus and Pseudomonas aeruginosa

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Last updated: 01 Jan 2025

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Abstract

Massoia aromatica Becc. (Lauraceae) is a native plant of the eastern part of Indonesia. The bark contains an essential oil which has been reported to be a potential antiinfective agent against single species microbes. This research aimed to explore the potency of the massoia oil against biofilm of dual-species culture, consisted of Pseudomonas aeruginosa and Staphylococcus aureus. Pulverized massoia bark was steam-hydrodistilled to gain the essential oil (massoia oil). The oil was subjected to microdilution assay on dual-species cultures of P. Aeruginosa and S. aureus to observe the effects on the biofilm formation and degradation. Crystal violet 1% was used to stain the biofilm of which the optical density was observed by microplate reader at 540 nm. Scanning Electron Microscope (SEM) was used for ultrastructure observation of the cultures following sample application. Results showed that the essential oil caused the inhibition of the biofilm formation and degraded the preformed biofilm cultures tested in a concentration dependant manner . SEM revealed that the massoia oil caused cell ruptured of the P. aeruginosa. No observed changes in the S. aureus cells but less population density occurred. The massoia oil showed effective inhibition towards dual-species biofilm.

DOI

10.21608/ejchem.2022.169290.7100

Keywords

Biofilm, Massoia aromatica Becc, P. aeruginosa, S. aureus Scanning Electron Microscope

Authors

First Name

Mentarry

Last Name

Bafadala

MiddleName

-

Affiliation

Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, 75119 East Kalimantan, Indonesia

Email

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City

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Orcid

-

First Name

Triana

Last Name

Hertiani

MiddleName

-

Affiliation

Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, 55281 Yogyakarta, Indonesia, Center of Natural Anti-infective Research, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, 55281 Yogyakarta, Indonesia, Biofilm Research Collaboration Centre, UGM-BRIN, Jl. Farmako Sekip Utara, 55281 Yogyakarta, Indonesia

Email

hertiani@ugm.ac.id

City

Yogyakarta

Orcid

0000-0002-1756-2478

First Name

Sylvia Utami Tunjung

Last Name

Pratiwi

MiddleName

-

Affiliation

Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, 55281 Yogyakarta, Indonesia, Center of Natural Anti-infective Research, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, 55281 Yogyakarta, Indonesia, Biofilm Research Collaboration Centre, UGM-BRIN, Jl. Farmako Sekip Utara, 55281 Yogyakarta, Indonesia

Email

sylvia_pratiwi@ugm.ac.id

City

-

Orcid

-

First Name

Angga Cipta

Last Name

Narsa

MiddleName

-

Affiliation

Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Mulawarman, Samarinda, 75119 East Kalimantan, Indonesia

Email

-

City

-

Orcid

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Volume

66

Article Issue

8

Related Issue

42250

Issue Date

2023-08-01

Receive Date

2022-10-17

Publish Date

2023-08-01

Page Start

185

Page End

190

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 8_Pages 185-190.pdf

PDF . 436.1kB

Link

https://ejchem.journals.ekb.eg/article_272029.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=272029

Order

272,029

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Exploring The Inhibitory Potential of Massoia Oil on Biofilm Dual-Species Culture Of Staphylococcus aureus and Pseudomonas aeruginosa

Details

Type

Article

Created At

30 Dec 2024