Pouteria campechiana (Kunth) Baehni is native to America and is cultivated in Egypt for its edible fruit. UPLC-PDA-qTOF-MS technique was performed to detect the metabolites profile of three organs of the Egyptian plant, namely, leaves, seeds and pericarps. UPLC-MS spectra was analyzed using Principal Component Analysis to explore the relative variability within organs. The antioxidant and antimicrobial activities of the ethanol extracts were assessed by free radical 1,1-diphenyl-2-picrylhydrazyl scavenging activity and agar well diffusion assay methods, respectively. The IC50 values of the leaves, seeds and pericarps ethanol extracts were 73.68±8.12, 235.615±10.3 and 858.952±18.16 μg/mL, respectively. They showed antimicrobial activity against three bacterial and three fungal strains out of the eight tested microorganisms. Sixty-three chromatographic peaks were detected among organs. Two main principle components (PC1 and PC2) were responsible for the discrimination of the three organs. Flavonol glycosides were found highest in the leaves. Taxifolin and ursolic acid were predominant in the seeds. Unsaturated fatty acids contributed the most in the discrimination of pericarps from leaves and seeds.