Considerable portions of used chemical fertilizers have to be substituted with more ecofriendly sources of nutrients; due to their polluting impacts on the long term of application; bio-fertilizers are promising alternatives providing nutrients in available forms with almost no hazardous effects on soil. In this study, Bacillus amyloliquefaciens and Pseudomonas fluorescens were evaluated as plant growth promoting Rhizobacteria (PGPRs) to be recommended as efficient bio-fertilizers individually and/or in mixtures. Biochemical parameters including growth promoting traits and extracellular enzymes were quantitatively determined in bacterial cultures. Phosphatases, urease, Phosphate solublization and Indole-acetic acid (IAA) and gibbrillic acid (GA) production were the most important parameters measured. Results showed that B. amyloliquefaciens was extremely distinguished in producing alkaline and acidic phosphatases reached almost 21 and 16 enzymatic units, respectively. As a growth promoting bacterium, B. amyloliquefaciens produced about 53 µmole GA/ml and 640 nmole IAA/ml. On the contrary, P. fluorescens was more efficient in inorganic phosphate souliblization and urease production than B. amyloliquefaciens. Urease units produced by P. fluorescens were up to 160 (U). In view of that, B. amyloliquefaciens and Pseudomonas fluorescens could be recommended to be applied as bioertilizers.