The aim of this study was to determine the physiological and histological changes in the liver and kidneys induced by glyphosate exposure in male albino rats and the ameliorative effects of broccoli and cauliflower, as well as their role in stimulating the liver to produce glutathione. The chemical composition of broccoli and cauliflower was determined, and the results showed the presence of natural antioxidants. Thirty- six animals were used for the study. NC- negative control group (received distilled water). PC (positive control group) -were used for the LD10, which was evaluated to be 375 mg/kg glyphosate. GBH + GSH group -glyphosate 375 mg/kg and glutathione 50 mg/kg b. wt. GBH+ Broccoli group- glyphosate 375 mg/kg and broccoli 5 g/kg b. wt. GBH+ Cauliflower group- glyphosate 3750 mg/kg and cauliflower 5 mg/kg b. wt. GBH+ Broccoli+ Cauliflower group- glyphosate 375 mg/kg, broccoli 2.5 mg/kg b. wt. and cauliflower 2.5 mg/kg b. wt. for eight weeks. The levels of antioxidant enzymes and glutathione in the liver, kidney, and serum were measured at the end of the experiment. According to our findings, glutathione peroxidase and glutathione – s transferase were enhanced in positive groups, whereas reduced glutathione was decreased. In the positive group, liver enzymes were significantly different from those in the negative control group and kidney enzymes in the positive group were considerably different from those in the negative groups. But when broccoli and cauliflower were given, this led to a significant improvement in both liver and kidney functions. Also, oral administration of glutathione has been demonstrated to reduce the toxic effect of glyphosate and improve liver and kidney function, although glutathione can be synthesized in the liver which resulted in a reduction in the negative effects of glyphosate. Histological changes in the liver and kidneys were determined which confirmed what the physiological results showed. In conclusion, Glyphosate exposure was observed to cause toxicity in rats' hepatic and renal functions, which was exacerbated by broccoli and cauliflower consumption, which in turn stimulates the production of glutathione.