In this study, the growth rate of Aglaonema plants (Aglaonema commutatum) was measured by applying different levels of brassinolide at (50, 100, and 200 ppm) and chitosan at (250, 500, and 1000 ppm) using foliar spray application. The obtained results showed that brassinolide at (200 ppm) foliar application affected significantly (number of leaves and root length). However, chitosan at (250 ppm) foliar application was more effective than brassinolide foliar application on plant growth parameters (plant height, stem diameter, total fresh and dry weights, and leaf area). Furthermore, chitosan at (250 ppm) foliar application increased the chemical parameters of Aglaonema plant (total chlorophylls, carotenoids, nitrogen, potassium, phenols, and indoles), and chitosan at (500 ppm) foliar application increased (phosphorus, phenols, and indoles). Both brassinolide at (200 ppm) and chitosan at (500 ppm) foliar application significantly increased (roots fresh and dry weights) of Aglaonema plant. The anatomical measurements of plants treated with chitosan foliar application showed higher values compared to plants treated with brassinolide foliar application and untreated plants.