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263866

In Vitro Propagation and Evaluation of Valtrate and Valereinic Acid in Valerina Officinalis L.

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Organic chemistry

Abstract

Valeriana officinalis L is the most cost-effective plant in Europe. Due to its cardiovascular, anxiolytic, sedative, and anticonvulsant properties, the root of Valeriana is extensively employed in herbal therapies. Seeds of Valeriana were germinated in vitro by soaking for 24 hours in 150 ppm Gibberellic acid (GA3) further, cultivated in half MS (Murashige and Skoog salts medium with vitamins)medium. Treatment of MS media with 5 ppm of Benzyl amino purine (BA) and 2,4-Dichlorophenoxy acetic acid (2,4-D) increased fresh and dry weights of calli produced from leaf explants. However, supplemented MS media with 3 ppm BA and 1 ppm 2,4-D enhanced the efficiency rate of regeneration. On the other hand, augmentation of MS-medium with 0.1 ppm Indole-3-butyric acid(IBA), achieved of rooting process, while acclimatization was accomplished by using peat moss:vermiculite (1:1). Valerenic acid and valtrate were analyzed qualitatively and quantitatively by HPLC in mother plant, callus, regenerated shoots, roots, and Dormival medication which used as a control. The findings showed that regenerated shoots had the greatest concentration of valerenic acid across various extracts (3.80±0.82mg/g DW), whereas in vitro-derived roots from leaf explants contained the highest concentration of valtrate (3.82±0.23mg/g DW).

DOI

10.21608/ejchem.2022.151906.6603

Keywords

Valeriana officinalis L, Callus, Regeneration, Roots, Acclimatization, HPLC, Valerenic acid and valtrate

Authors

First Name

Iman

Last Name

El Sheikh

MiddleName

El Seody

Affiliation

Chemistry of Medicinal Plants Department, Division of Pharmaceutical and Drug Industries, National Research Centre, Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt.

Email

emanseody@gmail.com

City

-

Orcid

0000-0002-2291-3906

First Name

seham

Last Name

elhawary

MiddleName

Salah

Affiliation

department of pharmacognosy, faculty of pharmacy, Cairo university

Email

seham.elhawary@pharma.cu.edu.eg

City

Cairo

Orcid

0000-0002-6823-1142

First Name

Soad

Last Name

Tadros

MiddleName

Hanna

Affiliation

Pharmacognosy Department, Faculty of Pharmacy. Cairo University

Email

soad.tadros@pharma.cu.edu.eg

City

-

Orcid

0009-0009-6533-7214

First Name

Naglaa

Last Name

Nazif

MiddleName

Mohamed

Affiliation

aChemistry of Medicinal Plants Department, Division of Pharmaceutical and Drug Industries, National Research Centre, Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt.

Email

naglaanazif@yahoo.com

City

-

Orcid

-

First Name

Mona

Last Name

AbdelMohsen

MiddleName

Mohamed

Affiliation

aChemistry of Medicinal Plants Department, Division of Pharmaceutical and Drug Industries, National Research Centre, Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt.

Email

monaamohsen@yahoo.com

City

-

Orcid

0000-0002-9927-2246

First Name

Hussein

Last Name

Taha

MiddleName

S.

Affiliation

Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Dokki, Cairo, Egypt

Email

hussein.taha2@yahoo.com

City

-

Orcid

0000-0003-0127-3425

Volume

66

Article Issue

7

Related Issue

42039

Issue Date

2023-07-01

Receive Date

2022-07-25

Publish Date

2023-07-01

Page Start

381

Page End

391

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 7_Pages 381-391.pdf

PDF . 823.1kB

Link

https://ejchem.journals.ekb.eg/article_263866.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=263866

Order

263,866

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

In Vitro Propagation and Evaluation of Valtrate and Valereinic Acid in Valerina Officinalis L.

Details

Type

Article

Created At

30 Dec 2024