Micro-organisms are the causes of many diseases, so we find a lot of research in the field of studying the biological efficacy of compounds on various types of pathogenic bacteria. Nowadays there are increasing numbers of infections caused by bacteria that are resistant to most of the antibacterial treatments currently available, for this causes we prepared series tetrazole derivative from different heterocyclic compounds like (tetrazole , thiadiazole, oxadiazole, oxazole, imidazole, triazole, thiazole . General studies have been steered for the derivatives to conclude their chemical configurations via spectroscopic performances (FT.IR ,H.NMR ,Mass)–spectra ,also Biotic Evaluation , Nano- study and other laboratory measurements. Some cyclic compounds are used as antimicrobials, and this is what we have proven in our research, as it has been proven that they are compounds that kill microorganisms or stop their growth. Antimicrobial drugs can be divided according to the microorganisms that they can work against. For example, antibiotics are used against bacteria and antifungals are used against fungi