Carbon nanotubes (CNTs) have been employed alone or in composites with other substances to generate highly useful materials ‎in a variety of industries. They are especially promising as scaffolds for bone tissue regeneration. To the best of our knowledge, ‎no studies have been published on the usage of lysine (Lys) or histidine (His) for functionalizing oxidised Multiwalled carbon ‎nanotubes (ox-MWCNTs) using the sol-gel process. The goal of this research is to create innovative hybrid composites for ‎biomedical applications based on ox-MWCNTs incorporating tetra ethyl orthosilicate (TEOS) and various amino acids (Lys or ‎His). Fourier transform infrared spectroscopy (FTIR), dynamic light scattering technique (DLS), X-ray diffraction (XRD), ‎thermogravimetric analysis (TGA), scanning and transmission electron microscopes (SEM and TEM) were used to characterise ‎the physicochemical properties.. In vitro bone bioactivity was tested after 7 days of soaking in simulated body fluid (SBF) at 37 ‎‎°C, as well as cytotoxicity against mice bone marrow stromal cells using the MTT assay. The results showed that both ‎functionalized MWCNTs had particle sizes of 1332 and 1329 nm and are colloidal stable. After soaking for 7 days, SEM ‎pictures revealed aggregated ca-apatite layers on the surface. The calcium element and the liberated free amino acids in the ‎residual SBF were approximately (2.1 and 0.9) and (2.6 and 2.0) mg/L in the case of Lys and His, respectively.‎ It can be ‎concluded that both covalent attached amino acids play enhancement role for the ca-apatite formation and lowering the toxicity in ‎the hybrid composites relative to the ox-MWCNTs especially in the case of His.‎