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257981

Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Food Chemistry

Abstract

Protein and fatty acid deficiency in human nutrition is a major concern for developing countries. The filamentous blue-green microalga Spirulina platensis is one of the best algal species that can be used as a food supplement. This study aims to optimize biomass, the total protein, and the fatty acid content of Spirulina platensis. Statistics optimization was carried out using the response surface methodology via dry weight and the Kjeldahl method for protein determination, while the fatty acid composition was determined by the conversion of oil to fatty acid methyl esters (FAMEs), which was measured on a gas chromatography (GC) device. The results revealed that the optimum conditions for maximum biomass, total protein, and fatty acid content were in the presence of NaHCO3 at (16.8 g/l) and NaNO3 (2.5 g/l) at temperature (20°C), pH (9), and LI (6000 lux). A prospective study to estimate both essential amino acids and essential fatty acids in S. platensis biomass is recommended.

DOI

10.21608/ejchem.2022.155468.6711

Keywords

Biomass, fatty acids, proteins, Response surface methodology, Spirulina platensis

Authors

First Name

Ahmad

Last Name

Badawy

MiddleName

Muhammad

Affiliation

Radiation Microbiology, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority , Cairo, Egypt

Email

ahmedbadawy22@hotmail.com

City

-

Orcid

-

First Name

Abeer

Last Name

Zakaria

MiddleName

Emam

Affiliation

Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority.

Email

abeer.mercy@yahoo.com

City

Cairo

Orcid

-

First Name

Ehab

Last Name

EI-Belely

MiddleName

Fathy

Affiliation

Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.

Email

elbelely@gmail.com

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

El-Gamal

MiddleName

Darwish

Affiliation

Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.

Email

ahmed46da@yahoo.co

City

Cairo

Orcid

-

First Name

Ali

Last Name

Hammad

MiddleName

Ahmed

Affiliation

Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority

Email

ahammad50@yahoo.co.uk

City

Cairo

Orcid

-

Volume

66

Article Issue

6

Related Issue

41553

Issue Date

2023-06-01

Receive Date

2022-08-10

Publish Date

2023-06-01

Page Start

217

Page End

223

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 66_Issue 6_Pages 217-223.pdf

PDF . 786.7kB

Link

https://ejchem.journals.ekb.eg/article_257981.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=257981

Order

23

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.

Details

Type

Article

Created At

30 Dec 2024