The development of green nanotechnology become very interest among researchers in order to synthesis eco-friendly of bio-nanoparticles. The current work used Trogoderma granarium larval extract as both a capping and reducing agent, as well as water as a solvent to biosynthesise stable silver nanoparticles (AgNPs). These biosynthesized nanoparticles were characterized with the help of atomic absorption spectroscopy (AAS UV–vis spectrophotometer), Zeta potential, Fourier infrared spectroscopy (FTIR), and atomic forced microscopy (AFM). It has been noted that within 5 minutes of reaction time, T. granarium larval extract can convert silver ions to silver nanoparticles. The antimicrobial activity of the AgNPs was screened against clinical isolate of multidrug-resistant Salmonella Typhi and showed inhibiting activities against Salmonella Typhi. As a result, this process may be utilized to produce rapid and eco-friendly stable silver nanoparticles with a size range of 59 nm which have antibacterial activity, suggesting that they could be exploited in the medical field.