Emerging and reemerging of viral outbreaks represent an ever-growing challenge to worldwide health. Nanoparticles have been attracting much interest due to their likely antiviral activity through interaction with viruses and are proposed to be one of the most promising solutions for detection, treatment, and prevention of viral diseases. Here, we report a gold nanoparticle conjugated extract from Streptomyces avermitilis shown to effectively inhibit hepatitis A [HAV], Adenovirus and Vesicular stomatitis virus [VSV]in cultured cells with half-maximal inhibitory concentrations of less than 4 nM and a selectivity index of greater than 550. A total of 20 strains of actinomycetes were isolated from agricultural soil for antiviral activity evaluation. Among which, [H1] exhibited a promising antiviral activity towards the three viruses. In an attempt to enhance the antiviral activity, Streptomyces extract was conjugated to 2.0 nm diameter gold nanoparticles. Although gold nanoparticles had low inhibitory effect on viral titer; the extract-gold nanoparticle conjugate displayed higher activity comparable to that of Streptomyces crude extract. This result support the hypothesis that multivalent molecules on the surface of gold nanoparticle can enhance potent therapeutics of active biological extracts due to interaction. We here introduce Streptomyces avermitilis H1 strain as a putative high antiviral producer with gold nanoparticle enhanced activity. More experimental effort on animal models needs to be performed to test for applicability of this developed conjugate in treatment of viral diseases.